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Quality assessment of AAV purified by resin tip and Robocolumn. Loading density was kept the same from each sample. (a) Full capsid percentage from mass photometry, (b) Purification recovery, (c) impurity level <t>(dsDNA)</t> and (d) HMW content after purification. Error bars correspond to standard deviation.
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Quality assessment of AAV purified by resin tip and Robocolumn. Loading density was kept the same from each sample. (a) Full capsid percentage from mass photometry, (b) Purification recovery, (c) impurity level <t>(dsDNA)</t> and (d) HMW content after purification. Error bars correspond to standard deviation.
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Quality assessment of AAV purified by resin tip and Robocolumn. Loading density was kept the same from each sample. (a) Full capsid percentage from mass photometry, (b) Purification recovery, (c) impurity level <t>(dsDNA)</t> and (d) HMW content after purification. Error bars correspond to standard deviation.
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Quality assessment of AAV purified by resin tip and Robocolumn. Loading density was kept the same from each sample. (a) Full capsid percentage from mass photometry, (b) Purification recovery, (c) impurity level <t>(dsDNA)</t> and (d) HMW content after purification. Error bars correspond to standard deviation.
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Quality assessment of AAV purified by resin tip and Robocolumn. Loading density was kept the same from each sample. (a) Full capsid percentage from mass photometry, (b) Purification recovery, (c) impurity level (dsDNA) and (d) HMW content after purification. Error bars correspond to standard deviation.

Journal: Biotechnology Progress

Article Title: Accelerating AAV purification process development using high‐throughput resin tip module

doi: 10.1002/btpr.70053

Figure Lengend Snippet: Quality assessment of AAV purified by resin tip and Robocolumn. Loading density was kept the same from each sample. (a) Full capsid percentage from mass photometry, (b) Purification recovery, (c) impurity level (dsDNA) and (d) HMW content after purification. Error bars correspond to standard deviation.

Article Snippet: The residual HC DNA content was evaluated using the Quanti‐iT PicoGreen double stranded DNA (dsDNA) assay from Thermo Fisher Scientific.

Techniques: Purification, Standard Deviation